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KMID : 0032220210330040339
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Annals of Dermatology
2021 Volume.33 No. 4 p.339 ~ p.344
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Potential Role of Cytosolic RNA Sensor MDA5 as an Inhibitor for Keratinocyte Differentiation in the Pathogenesis of Psoriasis
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Hong Dong-Kyun
Choi Mi-Ra
Hwang Yul-Lye
Lee Jae-Kyung
Lee Young
Seo Young-Joon
Kim Soo-Il
Lee Young-Ho
Kim Chang-Deok
Lee Jeung-Hoon
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Abstract
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Background: Psoriasis is a chronic inflammatory skin disease. The etiology of psoriasis is not fully understood, but the genetic background is considered to be the most important factor. To date, many psoriasis-related genes have been discovered, but the role of many important genes has not been well understood.
Objective: The purpose of this study is to uncover possible roles of MDA5 in psoriasis.
Methods: Expression of MDA5 was investigated using immunohistochemistry. Then, MDA5 was overexpressed in keratinocytes using a recombinant adenovirus.
Results: As a result of immunohistochemical staining, the expression of MDA5 was significantly increased in the epidermis of psoriasis compared to normal skin. Similarly, the expression of MDA5 was increased in imiquimod-induced psoriasiform dermatitis model. In cultured keratinocytes, toll-like receptor 3 agonist poly(I:C) induced expression of MDA5 at both mRNA and protein levels. When MDA5 was overexpressed using a recombinant adenovirus, poly(I:C)-induced cytokine expression was significantly increased. Finally, MDA5 overexpression significantly inhibited calcium-induced differentiation of keratinocytes.
Conclusion: These results suggest that MDA5 increases in psoriasis and negatively regulates keratinocyte differentiation.
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KEYWORD
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Differentiation, Keratinocytes, MDA5, Psoriasis
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